ABSTRACT
Streptococcus suis, a zoonotic bacterial pathogen circulated through swine, can cause severe infections in humans. Because human S. suis infections are not notifiable in most countries, incidence is underestimated. We aimed to increase insight into the molecular epidemiology of human S. suis infections in Europe. To procure data, we surveyed 7 reference laboratories and performed a systematic review of the scientific literature. We identified 236 cases of human S. suis infection from those sources and an additional 87 by scanning gray literature. We performed whole-genome sequencing to type 46 zoonotic S. suis isolates and combined them with 28 publicly available genomes in a core-genome phylogeny. Clonal complex (CC) 1 isolates accounted for 87% of typed human infections; CC20, CC25, CC87, and CC94 also caused infections. Emergence of diverse zoonotic clades and notable severity of illness in humans support classifying S. suis infection as a notifiable condition.
Subject(s)
Streptococcus suis , Humans , Animals , Swine , Molecular Epidemiology , Streptococcus suis/genetics , Europe/epidemiology , Phylogeny , Whole Genome SequencingABSTRACT
Strain HF14-78462T is an environmental bacterium found in clinical samples from an immunocompromized patient in 2014 at Hospital Universitari i Politècnic La Fe (Valencia, Spain). Phenotypically, strain HF14-78462T cells were Gram-stain-negative, aerobic, non-spore forming and non-motile small rods which formed mucous and whitish-translucent colonies when incubated at 20-36 °C. Phylogenetic analyses based on the 16S rRNA genes and the whole genomes of closest sequenced relatives confirmed that strain HF14-78462T is affiliated with the genus Starkeya. The strain was oxidase, catalase and urease positive; but indole, lysine decarboxylase, ornithine decarboxylase and DNase negative, did not produce H2S and was able to utilize a wide variety of carbon sources including acetamide, adonitol, amygdalin, l-arabinose, citric acid, glucose, mannitol and melibiose. Unlike Starkeya novella and Starkeya koreensis, strain HF14-78462T failed to grow in thiosulphate-oxidizing media and had a narrower temperature growth range. Its genome was characterized by a size of 4.83 Mbp and a C+G content of 67.75âmol%. Major fatty acids were C18:1 ω7c, cyclo C19â:â0 and C16â:â0, its polar acids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and an aminophospholipid; while the ubiquinones were Q9 (1.8â%) and Q10 (98.2â%). Digital DNA-DNA hybridization values were 41 and 41.4 against S. novella and S. koreensis, respectively, while average nucleotide identity values were around 84â%. Phenotypic, average nucleotide identity and phylogenomic comparative studies suggest that strain HF14-78462T is a new representative of the genus Starkeya and the name Starkeya nomas sp. nov. is proposed. The type strain is HF14-78462T (=CECT 30124T=LMG 31874T).
Subject(s)
Fatty Acids , Noma , Humans , Fatty Acids/chemistry , Phylogeny , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , DNA, Bacterial/genetics , Bacterial Typing Techniques , Base Composition , BacteriaABSTRACT
No disponible
Subject(s)
Humans , Male , Adult , Bacteremia/diagnosis , Bacteremia/etiology , Cupriavidus/isolation & purification , Colitis, Ulcerative/complications , Bacteremia/microbiology , Immunosuppressive Agents/therapeutic use , Gram-Negative Aerobic Rods and Cocci/isolation & purification , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Imipenem/therapeutic useSubject(s)
Anti-Bacterial Agents/pharmacology , Bacteremia/diagnosis , Chryseobacterium/isolation & purification , Colistin/pharmacology , Drug Resistance, Bacterial , Gram-Negative Bacterial Infections/diagnosis , Anti-Bacterial Agents/administration & dosage , Autistic Disorder/complications , Bacteremia/microbiology , Bacteremia/pathology , Child , Chryseobacterium/drug effects , Ciprofloxacin/administration & dosage , Female , Gram-Negative Bacterial Infections/microbiology , Gram-Negative Bacterial Infections/pathology , Humans , Treatment Outcome , Trimethoprim, Sulfamethoxazole Drug Combination/administration & dosageABSTRACT
Streptococcus pyogenes appears to be almost exclusively restricted to humans, with few reports on isolation from animals. We provide a detailed characterization (emm typing, pulsed-field gel electrophoresis [PFGE], and multilocus sequence typing [MLST]) of 15 S. pyogenes isolates from animals associated with different clinical backgrounds. We also investigated erythromycin resistance mechanisms and phenotypes and virulence genes. We observed 2 emm types: emm12 (11 isolates) and emm77 (4 isolates). Similarly, we observed 2 genetic linages, sequence type (ST) 26 and ST63. Most isolates exhibited the M macrolide resistance phenotype and the mefA/ermB genotype. Isolates were grouped into 2 clones on the basis of emm-MLST-PFGE-virulence gene profile combinations: clone 1, characterized by the combined genotype emm12-ST36-pulsotype A-speG; and clone 2, characterized by the genotype emm77-ST63-pulsotype B-speC. Our results do not show conclusively that animals may represent a new reservoir of S. pyogenes but indicate the ability of human-derived S. pyogenes isolates to colonize and infect animals.
Subject(s)
Drug Resistance, Bacterial/genetics , Genotype , Sheep Diseases/epidemiology , Streptococcal Infections/epidemiology , Streptococcal Infections/veterinary , Streptococcus pyogenes/genetics , Animals , Anti-Bacterial Agents/pharmacology , Antigens, Bacterial/genetics , Antigens, Bacterial/metabolism , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/metabolism , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Carrier Proteins/genetics , Carrier Proteins/metabolism , Clone Cells , Electrophoresis, Gel, Pulsed-Field , Farms , Gene Expression , Macrolides/pharmacology , Membrane Proteins/genetics , Membrane Proteins/metabolism , Microbial Sensitivity Tests , Multilocus Sequence Typing , Phenotype , Rabbits , Sheep/microbiology , Sheep Diseases/drug therapy , Sheep Diseases/microbiology , Sheep Diseases/transmission , Spain , Streptococcal Infections/drug therapy , Streptococcal Infections/transmission , Streptococcus pyogenes/classification , Streptococcus pyogenes/drug effects , Streptococcus pyogenes/isolation & purificationABSTRACT
BACKGROUND: Members of the genus Pseudonocardia have been widely reported and recovered from several ecosystems, such as soil samples and plant samples. Pseudonocardia bacteria colonize the microbial communities on the integument of fungus gardening ant species. We present the first documented case of Pseudonocardia carboxydivorans isolated in human cerebrospinal fluid (CSF). To the best of our knowledge, this is the first report of an human infection by P. carboxydivorans. CASE PRESENTATION: A patient, who suffered a traumatic brain injury a month before, was admitted to this hospital due to gait alteration and cognitive disturbances. Culture of cerebrospinal fluid showed ramified, not acid-fast, Gram positive bacilli. The bacterium was identified by molecular methods as P. carboxydivorans. CONCLUSION: This is the first documented case of isolating P. carboxydivorans in human CSF in a case of probable meningitis. Further research is needed in order to determine its pathogenic role in human infections.
Subject(s)
Actinobacteria/isolation & purification , Brain Injuries, Traumatic/complications , Gram-Positive Bacterial Infections/cerebrospinal fluid , Actinobacteria/genetics , Actinobacteria/pathogenicity , Aged , Brain Injuries, Traumatic/microbiology , Cerebrospinal Fluid/microbiology , Gram-Positive Bacterial Infections/drug therapy , Gram-Positive Bacterial Infections/etiology , Gram-Positive Bacterial Infections/microbiology , Humans , MaleABSTRACT
No disponible
Subject(s)
Humans , Male , Middle Aged , Peritonitis/complications , Peritonitis/diagnosis , Peritonitis/drug therapy , Streptococcus equi , Streptococcus equi/isolation & purification , Paracentesis/methods , Aztreonam/therapeutic use , Metronidazole/therapeutic use , Microbial Sensitivity Tests/methods , Levofloxacin/therapeutic use , Cholangiography , Constriction, Pathologic/complications , Constriction, PathologicABSTRACT
Christensenella minuta is a Gram-negative strictly anaerobic short rod that inhabits the human gut. This bacterium was isolated in a mixed infection with Desulfovibrio desulfuricansfrom the blood of a patient with a diagnosis of acute appendicitis. The strain was identified by 16S rRNA sequence analysis. As far as we know, this is the first time C.minuta has been isolated from a human clinical specimen.
Subject(s)
Appendicitis/diagnosis , Bacteremia/diagnosis , Clostridiales/isolation & purification , Coinfection/diagnosis , Gram-Positive Bacterial Infections/diagnosis , Adolescent , Appendicitis/complications , Appendicitis/microbiology , Bacteremia/microbiology , Bacteremia/pathology , Clostridiales/classification , Clostridiales/genetics , Coinfection/microbiology , Coinfection/pathology , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Desulfovibrio desulfuricans/classification , Desulfovibrio desulfuricans/genetics , Desulfovibrio desulfuricans/isolation & purification , Desulfovibrionaceae Infections/diagnosis , Desulfovibrionaceae Infections/microbiology , Desulfovibrionaceae Infections/pathology , Gram-Positive Bacterial Infections/microbiology , Gram-Positive Bacterial Infections/pathology , Humans , Male , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNAABSTRACT
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin --#91;1 mef(A), 3 erm(TR), 1 mef(A) + erm(TR) and 1 erm(TR) + erm(B)--#93; and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline --#91;2 tet(M), 5 tet(M) + tet(O)--#93;. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLS B phenotype.
Streptococcus dysgalactiae subsp. equisimilis (SDSE) posee factores de virulencia similares a Streptococcus pyogenes y, en consecuencia, produce faringitis e infecciones graves indistinguibles de las generadas por este patógeno clásico. Los objetivos del estudio fueron conocer la prevalencia de SDSE en infecciones invasivas en Argentina, estudiar su diversidad genética, determinar la presencia de genes de virulencia, ensayar su sensibilidad a los antibióticos y conocer los genes de resistencia. Se emplearon métodos convencionales de identificación. La sensibilidad se determinó por difusión, Etest y dilución en agar. Participaron 28 centros de 16 ciudades argentinas. Se obtuvieron 23 aislamientos (16 del grupo G y 7 del grupo C) desde el 1-7-2011 hasta el 30-6-2012. Se registraron 2 muertes en adultos (8,7%). La mayoría de los aislamientos fueron obtenidos de sangre (60,9%). Todos eran portadores de los genes speJ y ssa. Los genotipos más frecuentes fueron stG62647, stG653 y stG840. Se detectaron 19 pulsotipos distintos. Todos los aislamientos fueron sensibles a penicilina y levofloxacina, 6 (26,1%) presentaron resistencia o sensibilidad disminuida a eritromicina (1 mef--#91;A--#93;, 3 erm--#91;TR--#93;, 1 mef--#91;A--#93; + erm--#91;TR--#93; y 1 erm--#91;TR--#93; + erm--#91;B--#93;) y 7 (30,4%) fueron resistentes o tuvieron sensibilidad disminuida a tetraciclina (2 tet--#91;M--#93;, 5 tet--#91;M--#93; + tet--#91;O--#93;). La prevalencia anual en la Argentina fue de al menos 23 infecciones invasivas por SDSE y se observó una amplia diversidad genética. Todos los aislamientos presentaron los genes ssa y speJ. Como en otros estudios, la resistencia a macrólidos (26,1%) estuvo asociada, principalmente, al fenotipo MLS B.
Subject(s)
Humans , Male , Female , Streptococcal Infections/classification , Streptococcus/isolation & purification , Streptococcus/pathogenicity , Argentina , Streptococcus/genetics , Drug Resistance, Microbial , Cross-Sectional Studies/methodsABSTRACT
No disponible
Subject(s)
Humans , Male , Middle Aged , Bordetella/isolation & purification , Bordetella Infections/complications , Bordetella Infections/drug therapy , Bordetella Infections/pathology , Microbial Sensitivity Tests/methods , Microbial Sensitivity Tests , Cystic Fibrosis/complications , 51426 , Trimethoprim, Sulfamethoxazole Drug Combination/therapeutic use , Penicillins/therapeutic use , Cephalosporins/therapeutic use , Minocycline/therapeutic useABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Gordonia Bacterium , Gordonia Bacterium/isolation & purification , Abscess/complications , Abscess/diagnosis , Abscess/drug therapy , Amoxicillin-Potassium Clavulanate Combination/therapeutic use , Agar/analysis , Anti-Infective Agents/therapeutic use , Sputum/cytology , Sputum/microbiologyABSTRACT
Human infections caused by Streptococcus lactarius have not been previously reported. In the present report, we describe a lactational mastitis caused by this organism. The infection occurred in a 28-year-old breast-feeding female, with a 10-days history of moderate pain on the right breast. The patient was cured after antibiotic treatment with levofloxacin for 21 days. Our case shows that S. lactarius should be considered as a cause of lactational mastitis. The introduction of molecular microbiology techniques can be extremely useful for knowing the implication of streptococci in lactational mastitis.
Subject(s)
Mastitis/etiology , Mastitis/pathology , Streptococcal Infections/diagnosis , Streptococcal Infections/pathology , Streptococcus/classification , Streptococcus/isolation & purification , Adult , Anti-Bacterial Agents/therapeutic use , Female , Humans , Levofloxacin/therapeutic use , Mastitis/drug therapy , Mastitis/microbiology , Molecular Diagnostic Techniques , Streptococcal Infections/drug therapy , Streptococcal Infections/microbiology , Treatment OutcomeABSTRACT
No disponible
Subject(s)
Humans , Female , Middle Aged , Nocardia/isolation & purification , Nocardia Infections/microbiology , Injections, Intralesional/adverse effects , Osteoarthritis/drug therapyABSTRACT
Streptococcus dysgalactiae subsp. equisimilis (SDSE) has virulence factors similar to those of Streptococcus pyogenes. Therefore, it causes pharyngitis and severe infections indistinguishable from those caused by the classic pathogen. The objectives of this study were: to know the prevalence of SDSE invasive infections in Argentina, to study the genetic diversity, to determine the presence of virulence genes, to study antibiotic susceptibility and to detect antibiotic resistance genes. Conventional methods of identification were used. Antibiotic susceptibility was determined by the disk diffusion and the agar dilution methods and the E-test. Twenty eight centers from 16 Argentinean cities participated in the study. Twenty three isolates (16 group G and 7 group C) were obtained between July 1 2011 and June 30 2012. Two adult patients died (8.7%). Most of the isolates were recovered from blood (60.9%). All isolates carried speJ and ssa genes. stG62647, stG653 and stG840 were the most frequent emm types. Nineteen different PFGE patterns were detected. All isolates were susceptible to penicillin and levofloxacin, 6 (26.1%) showed resistance or reduced susceptibility to erythromycin [1 mef(A), 3 erm(TR), 1 mef(A)+erm(TR) and 1 erm(TR)+erm(B)] and 7 (30.4%) were resistant or exhibited reduced susceptibility to tetracycline [2 tet(M), 5 tet(M)+tet(O)]. The prevalence in Argentina was of at least 23 invasive infections by SDSE. A wide genetic diversity was observed. All isolates carried speJ and ssa genes. Similarly to other studies, macrolide resistance (26.1%) was mainly associated to the MLSB phenotype.
Subject(s)
Bacteremia/microbiology , Streptococcal Infections/microbiology , Streptococcus/genetics , Adolescent , Adult , Aged , Aged, 80 and over , Argentina/epidemiology , Arthritis, Infectious/epidemiology , Arthritis, Infectious/microbiology , Bacteremia/epidemiology , Body Fluids/microbiology , Child , Child, Preschool , DNA, Bacterial/genetics , Drug Resistance, Multiple, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Female , Genes, Bacterial , Genetic Variation , Humans , Infant , Male , Middle Aged , Prevalence , Soft Tissue Infections/epidemiology , Soft Tissue Infections/microbiology , Streptococcal Infections/epidemiology , Streptococcus/drug effects , Streptococcus/isolation & purification , Streptococcus/pathogenicity , Streptococcus pyogenes/genetics , Streptococcus pyogenes/isolation & purification , Virulence , Young AdultABSTRACT
BACKGROUND: We describe a case of traumatic ocular endophthalmitis caused by Nocardia kruczakiae after vegetable trauma in an immunocompetent child. FINDINGS: A 5-year-old boy suffered from a trauma with a palm tree leaflet. Two months later, he was diagnosed with traumatic infectious uveitis and intumescent cataract with anterior capsule rupture. Intensive treatment with systemic and topical vancomycin, ceftazidime and methylprednisolone began. After 1 month, he underwent phacoemulsification with intraocular lens implantation (IOL). After some episodes of reactivation, he was diagnosed with traumatic nocardial endophthalmitis from aqueous humour samples. Several operations and specific antibiotic therapy resolved the infection. CONCLUSIONS: In cases of traumatic endophthalmitis and several recurrences, it is extremely useful to make an etiologic diagnosis in order to treat the patient with specific antibiotics.
ABSTRACT
No disponible
